Journal: Antibiotics

Article Title: Pharmaceutical Prospects of Bee Products: Special Focus on Anticancer, Antibacterial, Antiviral, and Antiparasitic Properties

doi: 10.3390/antibiotics10070822

Figure Lengend Snippet: Antibacterial activity of selected bee products.

Article Snippet: Red propolis, green propolis, and brown propolis (traditionally extracted using ethanol or supercritical extraction method) , Brazil , S. aureus (ATCC 25923) and Enterococcus sp. (ATCC 29712), Klebsiella sp. (ATCC 1706/700603), and E. coli (ATCC 25922) , In vitro (micro-dilution assay) , Of all samples, Brazilian red propolis extract yielded the highest antibacterial activity. Green propolis extract demonstrated weak to moderate antibacterial activity for most samples and brown propolis extract did not yield any antibacterial effect against the tested strains. None of the examined samples was active against E. coli , [ ] .

Techniques: Activity Assay, Bacteria, Derivative Assay, In Vitro, Diffusion-based Assay, Dilution Assay, Microdilution Assay, Extraction, Transgenic Assay, Luciferase, Control, Biofilm Production Assay, Concentration Assay, SYBR Green Assay, Viability Assay, Membrane, Permeability, Isolation

Journal: ACS Infectious Diseases

Article Title: Insights into the Spectrum of Activity and Mechanism of Action of MGB-BP-3

doi: 10.1021/acsinfecdis.2c00445

Figure Lengend Snippet: Thermal melt curves of gDNA (salmon) and gDNA:MGB-BP-3 Complex. (A) Exemplar melt curve from one experimental repeat, visually representing the different melt curves of gDNA and the gDNA:MGB-BP-3 Complex. Data has been fitted with a Boltzmann distribution. (B) Melting temperatures of gDNA and gDNA:MGB-BP-3 Complex calculated from fitted Boltzmann distributions using OriginPro 2021. All values are an average for n = 4 experimental repeats with an error of ±1 °C.

Article Snippet: Bacterial genomic DNA (ATCC 43300, DSM 13661; ATCC 27853, DSM1117; ATCC 700603, DSM 26371; ATCC 25922, DSM 1103; ATCC 19606, DSM 30007; ATCC 51299, DSM 12956; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) or salmon genomic DNA (deoxyribonucleic acid sodium salt from salmon testes, D1626, Merck) dissolved in 1 mM phosphate buffer pH 7.4 (containing 0.27 mM potassium chloride, 13.7 mM sodium chloride) to a concentration of 100 μg/mL in SybrSafe (SYBR Safe DNA Gel Stain, ×10,000 in DMSO, S33102 Invitrogen) was used as supplied by the manufacturer in DMSO, and MGB-BP-3 was prepared as 10 mM stock in DMSO.

Techniques:

Journal: ACS Infectious Diseases

Article Title: Insights into the Spectrum of Activity and Mechanism of Action of MGB-BP-3

doi: 10.1021/acsinfecdis.2c00445

Figure Lengend Snippet: Remaining Fluorescence (%) of SybrSafe Probe upon Addition of MGB-BP-3 to gDNA a

Article Snippet: Bacterial genomic DNA (ATCC 43300, DSM 13661; ATCC 27853, DSM1117; ATCC 700603, DSM 26371; ATCC 25922, DSM 1103; ATCC 19606, DSM 30007; ATCC 51299, DSM 12956; Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) or salmon genomic DNA (deoxyribonucleic acid sodium salt from salmon testes, D1626, Merck) dissolved in 1 mM phosphate buffer pH 7.4 (containing 0.27 mM potassium chloride, 13.7 mM sodium chloride) to a concentration of 100 μg/mL in SybrSafe (SYBR Safe DNA Gel Stain, ×10,000 in DMSO, S33102 Invitrogen) was used as supplied by the manufacturer in DMSO, and MGB-BP-3 was prepared as 10 mM stock in DMSO.

Techniques: Fluorescence

Journal: Pharmaceutics

Article Title: Biosynthesis and Biological Properties of Nano-Silver from Aspergillus terreus Towards Antibacterial and Antitumor Applications

doi: 10.3390/pharmaceutics18020261

Figure Lengend Snippet: Antibacterial activity of biosynthesized AgNPs against Gram-positive and Gram-negative bacteria. ( A ) Representative images from the well diffusion assay showing inhibition zones for Gram-positive bacterial strains ( MRSA , MSSA , S. faecalis ) and Gram-negative bacterial strains ( E. coli , S. flexneri , S. enterica , P. vulgaris , K. quasipneumoniae , P. aeruginosa ). ( B – F ) MIC assay results for different bacterial strains, displaying a colourimetric indicator (pink for bacterial growth and blue for no growth) across a dilution series of AgNP concentrations. ( G ) Bar graph summarizing the MIC values for all tested bacterial strains, highlighting the higher sensitivity of Gram-negative bacteria ( P. aeruginosa and S. flexneri ) compared to Gram-positive bacteria (MRSA, MSSA).

Article Snippet: The antibacterial activity of AgNPs was assessed for both Gram-positive and Gram-negative bacterial strains, including Methicillin-resistant Staphylococcus aureus ( MRSA , ATCC 43300), Methicillin-sensitive Staphylococcus aureus ( MSSA , ATCC 25923), Klebsiella quasipneumoniae (ATCC 700603), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Shigella flexneri (ATCC 12022), Salmonella enterica (ATCC 13076), Proteus vulgaris (ATCC 49132), and Streptococcus faecalis (ATCC 19433).

Techniques: Activity Assay, Bacteria, Diffusion-based Assay, Inhibition